国产精品国产三级区别第一集,亚洲精品国产成人,精品人妻无码一区二区三区,人妻熟妇乱又伦精品视频

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>動物上皮細(xì)胞>CCL-64Mv.1.Lu(NBL-7) 貂肺上皮細(xì)胞

Mv.1.Lu(NBL-7) 貂肺上皮細(xì)胞

簡要描述:CCL-64 Mv.1.Lu(NBL-7) 貂肺上皮細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件
上海復(fù)祥生物公司現(xiàn)貨供應(yīng)美國典型培養(yǎng)物保藏中心(ATCC)各類細(xì)胞,*。 手機(jī) xiangfbio.歡迎各位老師來電咨!

  • 產(chǎn)品型號:CCL-64
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問  量:2923

產(chǎn)品分類

Product Category

詳細(xì)介紹

CCL-64 Mv.1.Lu(NBL-7) 貂肺上皮細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)優(yōu)培養(yǎng)條件

CCL-64 Mv.1.Lu(NBL-7) 貂肺上皮細(xì)胞 的詳細(xì)介紹

CCL-64

ATCC® Number:CCL-64™  Price:$275.00
Designations:Mv 1 Lu (NBL-7)

Depositors:AJ Kniazeff

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Musa vison (mink)

Morphology:epithelial


Source:Organ: lung

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Isolation:Isolation date: May, 1964

Applications:transfection host (Roche FuGENE® Transfection Reagents)

Virus Resistance:adenovirus 5; coxsackievirus A9, B5; poliovirus 2

Cytogenetic Analysis:Both male and female diploid cells as well as pseudodiploid cells are present. Approximay 58% of the cells have a chromosome number within + or - 1 of the diploid and one dicentric chromosome is present in some cells of the population., Both male and female diploid cells as well as pseudodiploid cells are present. Approximay 58% of the cells have a chromosome number within + or - 1 of the diploid and one dicentric chromosome is present in some cells of the population.

Age:near term fetus

Gender:male and female mixed

Comments:The Mv 1 Lu (NBL-7) cell line was initiated by A.J. Kniazeff, W.A. Nelson-Rees and N.B. Darby, Jr., in May, 1964, from trypsinized lungs of several nearly full-term, unsexed fetuses of the Aleutian mink.The cells are useful for focus forming assays for murine and feline sarcoma viruses [PubMed: 4366800].

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37C.


Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days


Preservation:Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

purified DNA:ATCC CCL-64D

0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101

Cell culture tested DMSO:ATCC 4-X



References:

















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
亚洲av乱码一区二区三区香蕉 | 午夜时刻免费入口| 麻豆国产精品色欲AV亚洲三区| 久久精品国产精品青草| 国产人成无码视频在线观看| 丝袜 亚洲 另类 欧美 变态| 亚洲第一第二第三第四第五| 欧美性巨大╳╳╳╳╳高跟鞋| 免费精品人在线二线三线区别| 久久人妻少妇嫩草AV无码| 最新69成人国产精品视频免费| 久久久久久AV无码免费看大片| 人妻少妇被粗大爽9797pw| 95免费视频在线观看| 久久精品人人做人人爽电影蜜月| 男人狂桶女人出白浆免费视频| 欧美13一14娇小性| 国产亚洲精品久久久久久久| 99亚洲精品自拍AV成人软件| 国产偷人爽久久久久久老妇APP| 亚洲国产综合久久久精品| 亚洲va韩国va欧美va| 18禁无码国内精品久久综合88 | 亚洲无码一区二区三区| 欧美私人情侣网站| 亚洲av无码乱码在线观看富二代| 久久夜色精品国产欧美乱| 国产无遮挡裸体免费视频在线观看| 少妇AV一区二区三区无码| 国产精品久久久久久久免费a片| 国产AV国片精品无套内谢无码| 亚洲 欧美 动漫 少妇 自拍| 成人禁片免费播放35分钟| 久久WWW免费人成看片| 国产婷婷一区二区三区| 色欲人妻AAAAAA无码| 99久久国产综合精品成人影院| 玩弄人妻少妇老师美妇厨房| 国产免费AV| 亚洲国产一区二区三区亚瑟| 亚洲成色在线综合网站|