国产精品国产三级区别第一集,亚洲精品国产成人,精品人妻无码一区二区三区,人妻熟妇乱又伦精品视频

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人正常細(xì)胞>CRL-2190 HK-2 人腎近曲小管上皮細(xì)胞.

CRL-2190 HK-2 人腎近曲小管上皮細(xì)胞.

簡(jiǎn)要描述:CRL-2190 HK-2 人腎近曲小管上皮細(xì)胞.,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-15
  • 訪  問(wèn)  量:1878

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-2190 HK-2 人腎近曲小管上皮細(xì)胞.

ATCC® Number:CRL-2190™    Price:$272.00
Designations:HK-2Depositors: RA ZagerBiosafety Level:2 [Cells Contain Papilloma viral DNA sequences ]Shipped:frozenMedium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiens (human)Morphology:epithelial Source:Organ: kidney, cortex Tissue: proximal tubule Cell Type: human papillomavirus 16 (HPV-16) transformedCellular Products:alkaline phosphatase; gamma glutamyltranspeptidase; leucine aminopeptidase; acid phosphatase; cytokeratin; alpha 3, beta 1 integrin; fibronectinPermits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.CRL-2190 HK-2 人腎近曲小管上皮細(xì)胞.Receptors:epidermal growth factor (EGF), expressedDNA Profile (STR):Amelogenin: X,Y CSF1PO: 13 D13S317: 9 D16S539: 11,12 D5S818: 12 D7S820: 10,11 THO1: 9 TPOX: 8,9 vWA: 17,18Age:*****Gender:maleComments:HK-2 (human kidney 2) is a proximal tubular cell (PTC) line derived from normal kidney. The cells were immortalized by transduction with human papilloma virus 16 (HPV-16) E6/E7 genes. The recombinant retrovirus vector pLXSN 16 E6/E7 containing the HPV-16 E6/E7 genes was used to transfect the ectotropic packaging cell line Psi-2. Virus produced by the Psi-2 cells was used to infect the amphotropic packaging cell line PA317 (see ATCC CRL-9078). Virus produced by the PA317 cells was used to transduce primary PTCs. Although pLXSN 16 E6/E7 also confers resistance to neomycin, selection in G418 was not used to isolate transduced clones. The cell line appears to be derived from a single cell based on Southern and FISH analysis. The E6/E7 genes are present in the HK-2 genome as determined by PCR. The cells retain a phenotype indicative of well differentiated PTCs. They are positive for alkaline phosphatase, gamma glutamyltranspeptidase, leucine aminopeptidase, acid phosphatase, cytokeratin, alpha 3,beta 1 integrin, and fibronectin. The cells are negative for factor VIII related antigen, 6.19 antigen and CALLA endopeptidase. HK-2 cells retain functional characteristics of proximal tubular epithelium such as Na+ dependent / phlorizin sensitive sugar transport and adenylate cyclase responsiveness to parathyroid, but not to antidiuretic hormone. The cells are capable of gluconeogenesis as evidenced by their ability to make and store glycogen. HK-2 cells are anchorage dependent. The cells will not grow in methylcellulose, soft agar or suspension. HK-2 cells can reproduce experimental results obtained with freshly isolated PTCs.Propagation:ATCC complete growth medium: The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with each of the two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF). To make the complete growth medium, you will need to add the following components to the base medium:0.05 mg/ml BPE - provided with the K-SFM kit5 ng/ml EGF - provided with the K-SFM kit. NOTE: Do not filter complete medium. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C Growth Conditions: Cell growth is dependent on epidermal growth factor. The cells should not be allowed to become confluent. Subculture at 80% of confluence.Subculturing:Protocol:Remove and discard culture medium.Briefly rinse the cell layer with 0.05% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximay 125 xg for 5 to 10 minutes.Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.Incubate cultures at 37°C.


CRL-2190 HK-2 人腎近曲小管上皮細(xì)胞.
















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
久久精品亚洲av无码四区毛片| 高清欧美videossexo| 日本特黄特色aaaaa大片免费| 欧美大屁股XXXX高跟欧美黑人| 京东一热本色道久久爱| 成人做爰免费观看| 精品伊人久久大香线蕉| 日本少妇高潮久久久久久| 狠狠色丁香婷婷综合久久97| 一本大道熟女人妻中文字幕在线 | 欧美| 欧美精品久久久久a片| 亚洲欧美日韩精品久久亚洲区| 国产精品视频一区二区三区不卡| 国产精品一区二区久久不卡| 歪歪漫画网免费版在线观看| 亚洲2022国产成人精品无码区| 伊人思思久99久女女精品视频| 亚洲国产精品自在自线观看| 丰满人妻跪趴高撅肥臀| 国产亚洲精品久久久久久禁果TV| 我的少妇邻居全文免费| 亚洲精品乱码久久久久久按摩| 中文无码一区二区三区在线观看| 无码人妻精品一区二区三区蜜桃| 三人交free性欧美| 免费看的www哔哩哔哩| 精品午夜福利在线观看| 亚洲精品午夜VA久久成人| 亚洲人妻| 国产偷V国产偷V国产高清| 久久精品国产亚洲AV成人| 亚洲色偷偷综合亚洲AV伊人| 两个人看的www视频免费完整版| 色婷婷AV一区二区三区仙踪林| 免费看污又色又爽又黄又脏小说 | 东京热人妻系列无码专区| 亚洲 欧美 自拍 另类 日韩 | 国内露脸少妇精品视频| 99亚洲精品自拍AV成人软件| a级毛片无码久久精品免费|